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KMID : 0881720180330020124
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 2 p.124 ~ p.130
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Analytical Method Development for Determination of Silymarin by LC-MS/MS for Related Health Functional Foods
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Oh Mi-Hyun
Lee Jin-Hee
Kim Sang-A
Kim Mee-Hye
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Abstract
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The Ministry of Food and Drug Safety (MFDS) is amending its test methods for the use of health functional foods (dietary food supplement), in order to establish regulatory standards and specifications in Korea. In this regard, we continue to pursue and perform our research on the analytical method development for the items being researched and reviewed. In this study, we have developed a sensitive and selective test method that could simultaneously separate and determinate six major bioactive flavonolignans in silymarin, which are based on the use of a liquid chromatographic-tandem mass spectrometry (LC-MS/MS). The standard calibration curves presented a linearity effect with the correlation coefficient (r
2) > 0.999. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 0.3~9.0 μg/L and 0.8~27.3 μg/L, respectively. The recovery results ranged between 96.2~98.6% at 3 different concentration levels, and its relative standard deviations (RSDs) were less than 5% as noted in this study. The proposed analytical method was characterized with a noted high resolution of the individual silymarin constituents, and the assay was fully validated as well. Our research can provide a significant scientific evidence that can be useful to amend the silymarin test method for the Health Functional Food Code.
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KEYWORD
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Silymarin , Analytical method , LC-MS/MS , Health functional food
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